Prof. Richard Fitzgerald

Biography

Education: BSc in Biochemistry (National University of Ireland)PhD protein chemistry/enzymology (National University of Ireland) Employment history:

Research Fellow - Food Science Department, North Carolina State University, USA & at the Food Biotechnology Centre, University College, Cork.
Senior Research Officer - Chemistry Department, Teagasc, Moorepark.
Lecturer, Senior Lecturer and Associate Professor - Department of Life Sciences, University of Limerick.
Professor (personal chair) of Food Protein-Derived Bioactive Peptides - Department of Biological Sciences, University of Limerick

Research interests:

Food protein chemistry/biochemistry.
Food protein ingredients (extraction of proteins from different sources - animal, plant and marine proteins).
Generation and characterisation of enzymatic hydrolysates of food proteins using microbial, mammalian and plant derived food-grade proteinase and peptidase preparations.
Characterisation of the functional properties of food proteins and their enzymatic hydrolysates, e.g., solubility, heat stability, emulsification, foaming, turbidity, viscosity properties.
Bioactive peptides/nutraceuticals, i.e., enzymatic generation, characterisation and identification (LC-MS/MS) of bioactive peptides having blood pressure reducing, serum glucose regulation, antioxidant, immune-modulatory, mineral binding, etc, properties.
LC-MS/MS-based characterisation of the hydrolytic specificity of different enzyme activities on purified food protein substrates.
Enzymatic cross-linking (using transglutaminase) of food proteins and their hydrolysates.
Role of microbial and mammalian exopeptidases in food protein hydrolysate debittering.

Research Interests

Food protein ingredients,Functional food ingredients/nutraceuticals,Food protein chemistry/biochemistry,Enzymatic hydrolysis of food proteins, Enzymatic cross-linking of food proteins, Purification and characterisation of starter culture-derived aminopeptidases, Role of aminopeptidases in food protein hydrolysate debittering.

Peer Reviewed Journals

2020

Identification and characterisation of peptides from a boarfish (Capros aper) protein hydrolysate displaying in vitro dipeptidyl peptidase-IV (DPP-IV) inhibitory and insulinotropic activity

Harnedy-Rothwell P.;McLaughlin C.;O'Keeffe M.;Le Gouic A.;Allsopp P.;McSorley E.;Sharkey S.;Whooley J.;McGovern B.;O'Harte F.;FitzGerald R. (2020) Identification and characterisation of peptides from a boarfish (Capros aper) protein hydrolysate displaying in vitro dipeptidyl peptidase-IV (DPP-IV) inhibitory and insulinotropic activity. Food Research International

Biography

Research Interests

Publications

Peer Reviewed Journals

Identification and characterisation of peptides from a boarfish (Capros aper) protein hydrolysate displaying in vitro dipeptidyl peptidase-IV (DPP-IV) inhibitory and insulinotropic activity

Application of in silico approaches for the generation of milk protein-derived bioactive peptides

Interfacial/foaming properties and antioxidant activity of a silkworm (Bombyx mori) pupae protein concentrate

Variable Glycemic Responses to Intact and Hydrolyzed Milk Proteins in Overweight and Obese Adults Reveal the Need for Precision Nutrition

A cell-based evaluation of a non-essential amino acid formulation as a non-bioactive control for activation and stimulation of muscle protein synthesis using ex vivo human serum.

Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages

Dipeptidyl peptidase IV (DPP-IV) inhibitory properties of a camel whey protein enriched hydrolysate preparation

Integration of high and low field H-1 NMR to analyse the effects of bovine dietary regime on milk metabolomics and protein-bound moisture characterisation of the resulting mozzarella cheeses during ripening

Identification of bioactive peptides from brewers’ spent grain and contribution of Leu/Ile to bioactive potency

Phycobiliproteins, nitrogenous compounds and fatty acid contents in field-collected and cultured gametophytes of Porphyra dioica, a red sea vegetable

Assessment of the microstructural characteristics and the in vitro bioactive properties of sunflower oil-based emulsions stabilized by fava bean (vicia faba) protein

Role of carbohydrate conjugation on the emulsification and antioxidant properties of intact and hydrolysed whey protein concentrate

Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP-1 secretory activity in vitro and acute glucose lowering effects in mice

Exploring the use of a modified high-temperature, short-time continuous heat exchanger with extended holding time (hTST-EHT) for thermal inactivation of trypsin following selective enzymatic hydrolysi....

Peptide identification from a: Porphyra dioica protein hydrolysate with antioxidant, angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory activities

Generation of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides during the enzymatic hydrolysis of tropical banded cricket (Gryllodes sigillatus) proteins

Chemical constituents, antioxidant and anti-lipase activity of some wines produced in Georgia

Mung bean proteins and peptides: nutritional, functional and bioactive properties

In Silico Approaches Applied to the Study of Peptide Analogs of Ile Pro-Ile in Relation to Their Dipeptidyl Peptidase IV Inhibitory Properties

Atlantic salmon (Salmo salar) co-product-derived protein hydrolysates: A source of antidiabetic peptides

Fourier transform infrared spectral analysis in tandem with P-31 nuclear magnetic resonance spectroscopy elaborates detailed insights into phosphate partitioning during skimmed milk microfiltration and diafiltration

Identification of novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in camel milk protein hydrolysates

Functional properties of bovine milk protein isolate and associated enzymatic hydrolysates

Casein Hydrolysate with Glycemic Control Properties: Evidence from Cells, Animal Models, and Humans

Whey protein hydrolysate induced modulation of endothelial cell gene expression

Use of P-31 NMR and FTIR to investigate key milk mineral equilibria and their interactions with micellar casein during heat treatment

Blue whiting (Micromesistius poutassou) muscle protein hydrolysate with in vitro and in vivo antidiabetic properties

Angiotensin converting enzyme and dipeptidyl peptidase-IV inhibitory activities of transglutaminase treated sodium caseinate hydrolysates

Impact of enzyme inactivation conditions during the generation of whey protein hydrolysates on their physicochemical and bioactive properties

Bitterness in sodium caseinate hydrolysates: role of enzyme preparation and degree of hydrolysis

Aqueous and enzyme-extracted phenolic compounds from brewers' spent grain (BSG): Assessment of their antioxidant potential

Identification of angiotensin converting enzyme inhibitory and antioxidant peptides in a whey protein concentrate hydrolysate produced at semi-pilot scale

Response surface methodology applied to the generation of casein hydrolysates with antioxidant and dipeptidyl peptidase IV inhibitory properties

Isolation of peptides from a novel brewers spent grain protein isolate with potential to modulate glycaemic response

Generation of wheat gluten hydrolysates with dipeptidyl peptidase IV (DPP-IV) inhibitory properties

Fractionation and identification of antioxidant peptides from an enzymatically hydrolysed Palmaria palmata protein isolate

Effects of depleting ionic strength on P-31 nuclear magnetic resonance spectra of micellar casein during membrane separation and diafiltration of skim milk

Peptide identification in a salmon gelatin hydrolysate with antihypertensive, dipeptidyl peptidase IV inhibitory and antioxidant activities

Milk protein isolate (MPI) as a source of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides

Peptide composition and dipeptidyl peptidase IV inhibitory properties of beta-lactoglobulin hydrolysates having similar extents of hydrolysis while generated using different enzyme-to-substrate ratios

Seasonal variation in nitrogenous components and bioactivity of protein hydrolysates from Porphyra dioica

Bioactive peptides from Atlantic salmon (Salmo salar) with angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory, and antioxidant activities

Release of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from milk protein isolate (MPI) during enzymatic hydrolysis

Peptide identification in a porcine gelatin prolyl endoproteinase hydrolysate with angiotensin converting enzyme (ACE) inhibitory and hypotensive activity

Unlocking the biological potential of proteins from edible insects through enzymatic hydrolysis: A review

Purification and identification of antioxidant peptides from gelatin hydrolysate of seabass skin

Marine Processing Proteinaceous By-Products: A Source of Biofunctional Food Ingredients

Strategies for the discovery, identification and validation of milk protein-derived bioactive peptides

Evaluation of the antioxidant capacity of a milk protein matrix in vitro and in vivo in women aged 50-70 years

Angiotensin Converting Enzyme and Dipeptidyl Peptidase-IV Inhibitory, and Antioxidant Activities of a Blue Mussel (Mytilus edulis) Meat Protein Extract and Its Hydrolysates

Impact of enzyme preparation and degree of hydrolysis on peptide profile and nitrogen solubility of sodium caseinate hydrolysates

Strategies for the release of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in an enzymatic hydrolyzate of alpha-lactalbumin

Evaluation of the antioxidant capacity of a milk protein matrix in vitro and in vivo in women aged 50-70 years

Enzymatic generation of whey protein hydrolysates under pH-controlled and non pH-controlled conditions: Impact on physicochemical and bioactive properties

A casein hydrolysate protects mice against high fat diet induced hyperglycemia by attenuating NLRP3 inflammasome-mediated inflammation and improving insulin signaling

Structure activity relationship modelling of milk protein-derived peptides with dipeptidyl peptidase IV (DPP-IV) inhibitory activity

Learnings from quantitative structure-activity relationship (QSAR) studies with respect to food protein-derived bioactive peptides: a review

Prospects for the management of type 2 diabetes using food protein-derived peptides with dipeptidyl peptidase IV (DPP-IV) inhibitory activity

In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates

The effect of consuming Palmaria palmata-enriched bread on inflammatory markers, antioxidant status, lipid profile and thyroid function in a randomised placebo-controlled intervention trial in healthy adults

Antioxidant activity of co-products from milk fat processing and their enzymatic hydrolysates obtained with different proteolytic preparations

Comparison of extraction methods for selected carotenoids from macroalgae and the assessment of their seasonal/spatial variation

Purification and identification of dipeptidyl peptidase (DPP) IV inhibitory peptides from the macroalga Palmaria palmata

Quantitative analysis of bovine beta-casein hydrolysates obtained using glutamyl endopeptidase

In vitro assessment of the multifunctional bioactive potential of Alaska pollock skin collagen following simulated gastrointestinal digestion

Relative quantitation analysis of the substrate specificity of glutamyl endopeptidase with bovine alpha-caseins

Immunomodulatory potential of a brewers' spent grain protein hydrolysate incorporated into low-fat milk following in vitro gastrointestinal digestion

Profiling of the Molecular Weight and Structural Isomer Abundance of Macroalgae-Derived Phlorotannins

Milk proteins as a source of tryptophan-containing bioactive peptides

In vitro bioactive properties of intact and enzymatically hydrolysed whey protein: targeting the enteroinsular axis

A study of the ability of bioactive extracts from brewers' spent grain to enhance the antioxidant and immunomodulatory potential of food formulations following in vitro digestion

Generation and identification of angiotensin converting enzyme (ACE) inhibitory peptides from a brewers' spent grain protein isolate

In vitro bioactive properties of intact and enzymatically hydrolysed whey protein: targeting the enteroinsular axis

Extraction of antioxidant and ACE inhibitory peptides from Thai traditional fermented shrimp pastes

Utilisation of the isobole methodology to study dietary peptide-drug and peptide-peptide interactive effects on dipeptidyl peptidase IV (DPP-IV) inhibition

Bioactive properties of milk proteins in humans: A review

Substrate specificity of glutamyl endopeptidase (GE): hydrolysis studies with a bovine α-casein preparation.