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Dr. Patricia Armshaw

Biography

Patricia studied Industrial Biochemistry and graduated from the University of Limerick in 2008. On completion of her degree, she was awarded the Silver Medal for Academic Performance in the College of Science. She completed a Ph.D. in Molecular Microbiology in 2012. The focus of her work was on the analysis of a group of Mobile Genetic Elements termed the SXT/R391 family of Integrative Conjugative Elements. These mobile elements are known to spread antibiotic resistance determinants among gram-negative bacteria, specifically Vibrio cholerae and other enteric pathogens. On completion of her Ph.D., she then became involved in the DEMA project [http://www.dema-etoh.eu/en/]. The DEMA consortium aims to develop low cost technologies that allow for the direct production of bioethanol from microalgae. Within the consortium, she is primarily involved in the generation of a library of metabolically engineered high ethanol-producing microalgae strains. Patricia has recently joined the CES department as a lecturer in Industrial Biochemistry while she continues her work within the DEMA consortium.

Research Interests

Due to a rise in global CO2 levels and depletion of fossil fuel resources, biofuel energy solutions are of increasing importance. With the EU mandatory target of 10% substitution of fossil fuels by 2020, it is crucial that we rapidly find alternative cost effective sustainable solutions for biofuel production. With this in mind, my current research plans are focused on the production of 3rd generation biofuel solutions [biofuels derived from algae and cyanobacteria] which aim to directly convert solar energy to biofuel products. I am also interested in developing technologies that can utilise the spent cell biomass for environmentally beneficial applications and the optimisation of conversion processes for industrial waste materials to biodiesel and other bio-products.

Within the DEMA project, I am currently focused on the investigation of the utility of the cyanobacterial strain Synechocystis sp. PCC 6803 for the production of bioethanol. The utilisation of cyanobacteria for the production of biofuels has recently received significant attention due to the phototrophic nature of the organism and minimal requirement for land usage compared to 2nd generation biofuels. Synechocystis PCC 6803 is a model cyanobacterium for genetic manipulation and has been widely used to produce a range of biotechnological products such as isobutanol and lactic acid. We have integrated several versions of a non-native ethanol biosynthesis pathway into Synechocystis PCC 6803 and have generated a large library of ethanol-producing mutants. Under laboratory conditions, some of these strains produce levels of ethanol that are above that of the current state of the art. Currently, we are optimising these high-producing strains to further enhance ethanol productivity rates to improve the commercialisation potential of the DEMA process. To this end, a number of novel strategies are being investigated including the deletion of genes involved in storage compound accumulation and over-expression of pyruvate, the internal energy resource for ethanol production.

Professional Activities

Education

  • 2012 University of Limerick - PhD
  • 2008 University of Limerick - BSc (Hons)

Award

  • 2012 - BOC Gases Postgraduate Award
  • 2008 - Irish Research Council for Science, Engineering and Technology PhD research study grant
  • 2008 - Silver Medal for Academic Performance
  • 2005 - UREKA scholarship

Publications

Book Chapters

2015

The use of ACT as an educational tool for examining the spread of Antibiotic Resistance.
Michael P Ryan, Catherine C Adley, Patricia Armshaw and J Tony Pembroke
(2015) The use of ACT as an educational tool for examining the spread of Antibiotic Resistance.
In The Battle Against Microbial Pathogens: Basic Science, Technological Advances and Educational Programs ; Spain : Formatex

2015

The Potential of the Photoautotroph Synechocystis for Metal Bioremediation.
J. Tony Pembroke, Balakrishnan Naveena and Patricia Armshaw
(2015) The Potential of the Photoautotroph Synechocystis for Metal Bioremediation.
In Advances in Bioremediation of Wastewater and Polluted Soil; Croatia : In Tech

2015

UV stress responsive genes associated with enterobacterial Integrative Conjugative Elements of the ICE SXT/R391 group.
Armshaw, P., and J.T., Pembroke
(2015) UV stress responsive genes associated with enterobacterial Integrative Conjugative Elements of the ICE SXT/R391 group.
In Stress and Environmental control of Gene Expression in Bacteria; USA : Wiley/Blackwell

2013

Integrative Conjugative Elements (ICEs) of the SXT/R391 group as vehicles for acquisition of resistance determinants, stable maintenance and transfer to a wide range of enterobacterial pathogens
Armshaw, P. & Pembroke, J.T.
(2013) Integrative Conjugative Elements (ICEs) of the SXT/R391 group as vehicles for acquisition of resistance determinants, stable maintenance and transfer to a wide range of enterobacterial pathogens
In Microbial pathogens and strategies for combating them: science, technology and education ; Spain : Formatex

Peer Reviewed Journals

2015

Examination of the cell sensitizing gene orf43 of ICE R391 suggests a role in ICE transfer enhancement to recipient cells
Armshaw, Patricia,Pembroke, J. Tony
(2015) Examination of the cell sensitizing gene orf43 of ICE R391 suggests a role in ICE transfer enhancement to recipient cells
In Fems Microbiology Letters;

2015

Utilising the native plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC6803 as a cloning site for enhanced product production
Patricia Armshaw, Dawn Carey, Con Sheahan and J. Tony Pembroke
(2015) Utilising the native plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC6803 as a cloning site for enhanced product production
In Biotechnology for Biofuels;
DOI: 10.1186/s13068-015-0385-x
[ULIR]

2015

Ultrasonic intensification as a tool for enhanced microbial biofuel yields
Balakrishnan Naveena*, Patricia Armshaw and J. Tony Pembroke
(2015) Ultrasonic intensification as a tool for enhanced microbial biofuel yields
In Biotechnology for Biofuels; pp. 1-13
DOI: 10.1186/s13068-015-0321-0
[ULIR]

2015

The state of autotrophic ethanol production in Cyanobacteria
Dexter, J.,Armshaw, P.,Sheahan, C.,Pembroke, J. T.
(2015) The state of autotrophic ethanol production in Cyanobacteria
In Journal of Applied Microbiology;

2013

Control of expression of the ICE R391 encoded UV-inducible cell-sensitising function
Armshaw, Patricia,Pembroke, J. Tony
(2013) Control of expression of the ICE R391 encoded UV-inducible cell-sensitising function
In Bmc Microbiology;
[ULIR]

2013

Generation and analysis of an ICE R391 deletion library identifies genes involved in the element encoded UV-inducible cell-sensitising function
Armshaw, P.,Pembroke, J. T.
(2013) Generation and analysis of an ICE R391 deletion library identifies genes involved in the element encoded UV-inducible cell-sensitising function
In Fems Microbiology Letters; tony.pembroke@ul.ie :

Conference Publications

2015

Society for General Microbiology Irish Division Meeting: Microbial Interfaces NUI Galway
Lorraine Quinn , Patricia Armshaw , Dawn Carey , Con Sheahan and J. Tony Pembroke
(2015) Society for General Microbiology Irish Division Meeting: Microbial Interfaces NUI Galway

2015

Society for General Microbiology Irish Division Meeting: Microbial Interfaces NUI Galway
Dawn Carey , Patricia Armshaw , Lorraine Quinn , Con Sheahan and J. Tony Pembroke
(2015) Society for General Microbiology Irish Division Meeting: Microbial Interfaces NUI Galway

2015

Society for General Microbiology Irish Division Meeting: Microbial Interfaces NUI Galway
Naveena Balakrishnan, Patricia Armshaw, Con Sheahan, J. Tony Pembroke
(2015) Society for General Microbiology Irish Division Meeting: Microbial Interfaces NUI Galway

2015

Society for General Microbiology Irish Division Meeting: Microbial Interfaces
Kevin Hayes, Mohamed Noor, David Russell, Patricia Armshaw, J Tony Pembroke, Tewfik Soulimane
(2015) Society for General Microbiology Irish Division Meeting: Microbial Interfaces

2015

1st International Solar Fuels Conference (ISF-1)
Patricia Armshaw, Dawn Carey, Lorraine Quinn, Con Sheahan and J. Tony Pembroke
(2015) 1st International Solar Fuels Conference (ISF-1)

2015

Society for General Microbiology Irish Division Meeting: Microbial Interfaces NUI Galway
Kevin A. Hayes, Mohamed Noor, David Russell, Patricia Armshaw, J. Tony Pembroke and Tewfik Soulimane
(2015) Society for General Microbiology Irish Division Meeting: Microbial Interfaces NUI Galway

2014

Society for General Microbiology Irish Division meeting
Jason Dexter, Patricia Armshaw, Con Sheahan, J Tony Pembroke
(2014) Society for General Microbiology Irish Division meeting

2012

Society for General Microbiology Spring Conference 2012
Armshaw, P & Pembroke, J.T.
(2012) Society for General Microbiology Spring Conference 2012

2011

Proceedings of the Microscopy Society of Ireland Annual Symposium, CRANN, Trinity College Dublin, Ireland
Patricia Armshaw, Anna Piterina, J Tony Pembroke
(2011) Proceedings of the Microscopy Society of Ireland Annual Symposium, CRANN, Trinity College Dublin, Ireland

2011

Society for General Microbiology Spring Conference 2011, Harrogate International Centre, Harrogate, UK,
Patricia Armshaw, J Tony Pembroke
(2011) Society for General Microbiology Spring Conference 2011, Harrogate International Centre, Harrogate, UK,

2009

Young microbiologist mini-symposium: microbe signalling, organisation and pathogenesis,
Patricia Armshaw, John O' Halloran, J Tony Pembroke
(2009) Young microbiologist mini-symposium: microbe signalling, organisation and pathogenesis,

Other Publications

2012

Functional genomic analysis of the UV-inducible ‘cytotoxic' gene from the ICE R391, an archetypal member of the SXT/R391 family of Integrative Conjugative Elements
Patricia Armshaw
(2012) Functional genomic analysis of the UV-inducible ‘cytotoxic' gene from the ICE R391, an archetypal member of the SXT/R391 family of Integrative Conjugative Elements