Dr. Padraigin Harnedy
Biography
Dr Pádraigín Harnedy-Rothwell joined the technical team as a Senior Technical Officer (STO) in October 2022. She obtained her PhD in Biotechnology from Waterford Institute of Technology (WIT), and she has since worked as a part-time lecturer at WIT, in the Forensic Science Laboratory located in Garda HQ in the Phoenix Park, Dublin and as a postdoctoral researcher in Teagasc Ashtown Food Research Centre, Dublin. She joined the Biological Science Department in UL in 2009 as a postdoctoral researcher in the Proteins and Peptides Research Group which is led by Prof Dick FitzGerald and worked in the group until her appointment as a STO. During her time in the Proteins and Peptides Research Group her research focused on marine-derived proteinaceous functional food ingredients. In particular, the mining for and characterisation of marine-derived peptides for the prevention and management of type II diabetes, cardiovascular disease and obesity. From her career to date Pádraigín has gained significant knowledge and practical experience in technologies related to microbiology, molecular biology, and food science.Research Interests
My primary research interest is in the mining of multifunctional bioactive peptides from marine protein sources that have applications as functional food ingredients for the prevention and management of diseases such as type 2 diabetes and cardiovascular disease.
In particular I am interested in the development of industrial relevant protocols for the extraction of proteins from marine sources including macroalgae, salmon by-products, boarfish and blue whiting, the targeted generation, characterisation and identification of bioactive peptides therefrom.
Peer Reviewed Journals
A Cell-Based Assessment of the Muscle Anabolic Potential of Blue Whiting (Micromesistius poutassou) Protein Hydrolysates"
Shekoohi, Niloofar, Miryam Amigo-Benavent, Guilherme Wesley Peixoto da Fonseca, Pádraigín A. Harnedy-Rothwell, Richard J. FitzGerald, and Brian P. Carson. (2023) A Cell-Based Assessment of the Muscle Anabolic Potential of Blue Whiting (Micromesistius poutassou) Protein Hydrolysates". International Journal Of Molecular Sciences
Blue Whiting (Micromesistius poutassou) Protein Hydrolysates Increase GLP-1 Secretion and Proglucagon Production in STC-1 Cells Whilst Maintaining Caco-2/HT29-MTX Co-Culture Integrity
Heffernan, S;Nunn, L;Harnedy-Rothwell, PA;Gite, S;Whooley, J;Giblin, L;FitzGerald, RJ;O'Brien, NM (2022) Blue Whiting (Micromesistius poutassou) Protein Hydrolysates Increase GLP-1 Secretion and Proglucagon Production in STC-1 Cells Whilst Maintaining Caco-2/HT29-MTX Co-Culture Integrity. BASEL : MDPI Marine Drugs
In vitro angiotensin-converting enzyme and dipeptidyl peptidase-IV inhibitory, and antioxidant activity of blue mussel (Mytilus edulis) byssus collagen hydrolysates.
Neves A.C., Harnedy-Rothwell P.A. & FitzGerald R.J. (2022) In vitro angiotensin-converting enzyme and dipeptidyl peptidase-IV inhibitory, and antioxidant activity of blue mussel (Mytilus edulis) byssus collagen hydrolysates. . European Food Research And Technology
Physicochemical, Nutritional and In Vitro Antidiabetic Characterisation of Blue Whiting (Micromesistius poutassou) Protein Hydrolysates
Harnedy-Rothwell, PA;Khatib, N;Sharkey, S;Lafferty, RA;Gite, S;Whooley, J;O'Harte, FPM;FitzGerald, RJ (2021) Physicochemical, Nutritional and In Vitro Antidiabetic Characterisation of Blue Whiting (Micromesistius poutassou) Protein Hydrolysates. BASEL : MDPI Marine Drugs
Protein hydrolysates from boarfish (Capros aper) and Atlantic salmon (Salmo salar) skin gelatin improve metabolic control in genetically obese diabetic (ob/ob) mice
Parthsarathy, V., McLaughlin, C. M., Sharkey, S. J., Harnedy-Rothwell, P. A., Lafferty, R. A., Allsopp, P. J., McSorley, E. M., FitzGerald, R. J., & O'Harte, F. P. (2021) Protein hydrolysates from boarfish (Capros aper) and Atlantic salmon (Salmo salar) skin gelatin improve metabolic control in genetically obese diabetic (ob/ob) mice. Journal Of Food Biochemistry
A Fish-Derived Protein Hydrolysate Induces Postprandial Aminoacidaemia and Skeletal Muscle Anabolism in an In Vitro Cell Model Using Ex Vivo Human Serum
Lees, MJ;Nolan, D;Amigo-Benavent, M;Raleigh, CJ;Khatib, N;Harnedy-Rothwell, P;FitzGerald, RJ;Egan, B;Carson, BP (2021) A Fish-Derived Protein Hydrolysate Induces Postprandial Aminoacidaemia and Skeletal Muscle Anabolism in an In Vitro Cell Model Using Ex Vivo Human Serum. BASEL : MDPI Nutrients
Macroalgal protein hydrolysates from Palmaria palmata influence the 'incretin effect' in vitro via DPP-4 inhibition and upregulation of insulin, GLP-1 and GIP secretion
McLaughlin, CM;Harnedy-Rothwell, PA;Lafferty, RA;Sharkey, S;Parthsarathy, V;Allsopp, PJ;McSorley, EM;FitzGerald, RJ;O'Harte, FPM (2021) Macroalgal protein hydrolysates from Palmaria palmata influence the 'incretin effect' in vitro via DPP-4 inhibition and upregulation of insulin, GLP-1 and GIP secretion. HEIDELBERG : SPRINGER HEIDELBERG European journal of nutrition :4439-4452
In Vitro and In Vivo Effects of Palmaria palmata Derived Peptides on Glucose Metabolism
Harnedy-Rothwell, P.A., McLaughlin, C.M., Le Gouic, A.V., Mullen, C, Parthsarath, V., Allsopp, P.J., McSorley, E.M., FitzGerald, R.J. and O'Harte, F.P.M. (2021) In Vitro and In Vivo Effects of Palmaria palmata Derived Peptides on Glucose Metabolism. International Journal Of Peptide Research And Therapeutics :1667-1676
Blue Whiting Protein Hydrolysates Exhibit Antioxidant and Immunomodulatory Activities in Stimulated Murine RAW264.7 Cells
Heffernan S, Harnedy-Rothwell PA, Gite S, Whooley J, Giblin L, FitzGerald RJ, O'Brien NM. (2021) Blue Whiting Protein Hydrolysates Exhibit Antioxidant and Immunomodulatory Activities in Stimulated Murine RAW264.7 Cells. Applied Sciences (Switzerland) :9762-
Effect of in vitro simulated gastrointestinal digestion on the antioxidant activity of the red seaweed Porphyra dioica
Pimentel F.B.;Cermeño M.;Kleekayai T.;Harnedy P.A.;FitzGerald R.J.;Alves R.C.;Oliveira M.B.P.P. (2020) Effect of in vitro simulated gastrointestinal digestion on the antioxidant activity of the red seaweed Porphyra dioica. Food Research International
Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed.
Cermeño M;Kleekayai T;Amigo-Benavent M;Harnedy-Rothwell P;FitzGerald RJ; (2020) Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed.. Electrophoresis
Enzymatic Modification of Porphyra dioica-Derived Proteins to Improve their Antioxidant Potential
Pimentel F.B.;Cermeño M.;Kleekayai T.;Harnedy-Rothwell P.A.;Fernandes E.;Alves R.C.;Beatriz P.P.O.M.;FitzGerald R.J. (2020) Enzymatic Modification of Porphyra dioica-Derived Proteins to Improve their Antioxidant Potential. Molecules
A Narrative Review of the Anti-Hyperglycemic and Satiating Effects of Fish Protein Hydrolysates and Their Bioactive Peptides.
Sharkey SJ;Harnedy-Rothwell PA;Allsopp PJ;Hollywood LE;FitzGerald RJ;O'Harte FPM; (2020) A Narrative Review of the Anti-Hyperglycemic and Satiating Effects of Fish Protein Hydrolysates and Their Bioactive Peptides.. Molecular Nutrition & Food Research
Identification and characterisation of peptides from a boarfish (Capros aper) protein hydrolysate displaying in vitro dipeptidyl peptidase-IV (DPP-IV) inhibitory and insulinotropic activity
Harnedy-Rothwell P.;McLaughlin C.;O'Keeffe M.;Le Gouic A.;Allsopp P.;McSorley E.;Sharkey S.;Whooley J.;McGovern B.;O'Harte F.;FitzGerald R. (2020) Identification and characterisation of peptides from a boarfish (Capros aper) protein hydrolysate displaying in vitro dipeptidyl peptidase-IV (DPP-IV) inhibitory and insulinotropic activity. Food Research International
Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP-1 secretory activity in vitro and acute glucose lowering effects in mice
Parthsarathy, V;McLaughlin, CM;Harnedy, PA;Allsopp, PJ;Crowe, W;McSorley, EM;FitzGerald, RJ;O'Harte, FPM (2019) Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP-1 secretory activity in vitro and acute glucose lowering effects in mice. HOBOKEN : WILEY International Journal Of Food Science And Technology :271-281
Bioactive Peptides from Fish Protein By-Products
Le Gouic A.V.;Harnedy P.A.;FitzGerald R.J. (2019) Bioactive Peptides from Fish Protein By-Products. Reference Series In Phytochemistry :355-388
Atlantic salmon (Salmo salar) co-product-derived protein hydrolysates: A source of antidiabetic peptides
Harnedy, PA;Parthsarathy, V;McLaughlin, CM;O'Keeffe, MB;Allsopp, PJ;McSorley, EM;O'Harte, FPM;FitzGerald, RJ (2018) Atlantic salmon (Salmo salar) co-product-derived protein hydrolysates: A source of antidiabetic peptides. AMSTERDAM : ELSEVIER SCIENCE BV Food Research International :598-606
Blue whiting (Micromesistius poutassou) muscle protein hydrolysate with in vitro and in vivo antidiabetic properties
Harnedy, PA,Parthsarathy, V,McLaughlin, CM,O'Keeffe, MB,Allsopp, PJ,McSorley, EM,O'Harte, FPM,FitzGerald, RJ (2018) Blue whiting (Micromesistius poutassou) muscle protein hydrolysate with in vitro and in vivo antidiabetic properties. Journal Of Functional Foods :137-145
Bioactive peptides from Atlantic salmon (Salmo salar) with angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory, and antioxidant activities
Neves, AC,Harnedy, PA,O'Keeffe, MB,FitzGerald, RJ (2017) Bioactive peptides from Atlantic salmon (Salmo salar) with angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory, and antioxidant activities. Food chemistry :396-405
Seasonal variation in nitrogenous components and bioactivity of protein hydrolysates from Porphyra dioica
Stack, J,Tobin, PR,Gietl, A,Harnedy, PA,Stengel, DB,FitzGerald, RJ (2017) Seasonal variation in nitrogenous components and bioactivity of protein hydrolysates from Porphyra dioica. Journal Of Applied Phycology :2439-2450
Fractionation and identification of antioxidant peptides from an enzymatically hydrolysed Palmaria palmata protein isolate
Harnedy, PA,O'Keeffe, MB,FitzGerald, RJ (2017) Fractionation and identification of antioxidant peptides from an enzymatically hydrolysed Palmaria palmata protein isolate. Food Research International :416-422
Peptide identification in a salmon gelatin hydrolysate with antihypertensive, dipeptidyl peptidase IV inhibitory and antioxidant activities
Neves, AC,Harnedy, PA,O'Keeffe, MB,Alashi, MA,Aluko, RE,FitzGerald, RJ (2017) Peptide identification in a salmon gelatin hydrolysate with antihypertensive, dipeptidyl peptidase IV inhibitory and antioxidant activities. Food Research International :112-120
Angiotensin Converting Enzyme and Dipeptidyl Peptidase-IV Inhibitory, and Antioxidant Activities of a Blue Mussel (Mytilus edulis) Meat Protein Extract and Its Hydrolysates
Neves, AC,Harnedy, PA,FitzGerald, RJ (2016) Angiotensin Converting Enzyme and Dipeptidyl Peptidase-IV Inhibitory, and Antioxidant Activities of a Blue Mussel (Mytilus edulis) Meat Protein Extract and Its Hydrolysates. Journal Of Aquatic Food Product Technology :1221-1233
Marine Processing Proteinaceous By-Products: A Source of Biofunctional Food Ingredients
Neves, AC,Harnedy, PA,FitzGerald, RJ,Dhillon, GS (2016) Marine Processing Proteinaceous By-Products: A Source of Biofunctional Food Ingredients. Protein Byproducts: Transformation From Environmental Burden Into Value-Added Products :63-86
The effect of consuming Palmaria palmata-enriched bread on inflammatory markers, antioxidant status, lipid profile and thyroid function in a randomised placebo-controlled intervention trial in healthy adults
Allsopp, P,Crowe, W,Bahar, B,Harnedy, PA,Brown, ES,Taylor, SS,Smyth, TJ,Soler-Vila, A,Magee, PJ,Gill, CIR,Strain, CR,Hegan, V,Devaney, M,Wallace, JMW,Cherry, P,FitzGerald, RJ,Strain, JJ,O'Doherty, JV,McSorley, EM (2016) The effect of consuming Palmaria palmata-enriched bread on inflammatory markers, antioxidant status, lipid profile and thyroid function in a randomised placebo-controlled intervention trial in healthy adults. European journal of nutrition :1951-1962
Extraction of antioxidant and ACE inhibitory peptides from Thai traditional fermented shrimp pastes
Kleekayai, T, Harnedy, PA, O'Keeffe, MB, Poyarkov, AA, CunhaNeves, A, Suntornsuk, W, FitzGerald, RJ (2015) Extraction of antioxidant and ACE inhibitory peptides from Thai traditional fermented shrimp pastes. Food Chemistry :441-447
Purification and identification of dipeptidyl peptidase (DPP) IV inhibitory peptides from the macroalga Palmaria palmata.
Harnedy PA, O'Keeffe MB, FitzGerald RJ (2015) Purification and identification of dipeptidyl peptidase (DPP) IV inhibitory peptides from the macroalga Palmaria palmata.. Food Chemistry :400-406
Fractionation and identification of Alaska pollock skin collagen-derived mineral chelating peptides.
Guo L, Harnedy PA, O'Keeffe MB, Zhang L, Li B, Hou H, FitzGerald RJ (2015) Fractionation and identification of Alaska pollock skin collagen-derived mineral chelating peptides.. Food Chemistry :536-542
In vitro assessment of the multifunctional bioactive potential of Alaska pollock skin collagen following simulated gastrointestinal digestion
Guo, LD,Harnedy, PA,Zhang, L,Li, BF,Zhang, ZH,Hou, H,Zhao, X,FitzGerald, RJ (2015) In vitro assessment of the multifunctional bioactive potential of Alaska pollock skin collagen following simulated gastrointestinal digestion. Journal Of The Science Of Food And Agriculture :1514-1520
The effect of time and origin of harvest on the in vitro biological activity of Palmaria palmata protein hydrolysates
Harnedy, PA,Soler-Vila, A,Edwards, MD,FitzGerald, RJ (2014) The effect of time and origin of harvest on the in vitro biological activity of Palmaria palmata protein hydrolysates. Food Research International :746-752
Food protein-derived chelating peptides: biofunctional ingredients for dietary mineral bioavailability enhancement
Guo, L; Harnedy, PA; Li, B; Hou, H; Zhang Z; Zhao, X; FitzGerald, RJ (2014) Food protein-derived chelating peptides: biofunctional ingredients for dietary mineral bioavailability enhancement. Trends In Food Science & Technology :92-105
Cardioprotective peptides from marine sources.
Harnedy, P.A. & FitzGerald, R.J. (2013) Cardioprotective peptides from marine sources.. Current Protein & Peptide Science :162-172
Extraction of protein from the macroalga Palmaria palmata
Harnedy, PA,FitzGerald, RJ (2013) Extraction of protein from the macroalga Palmaria palmata. Lwt-Food Science And Technology :375-382
In vitro assessment of the cardioprotective, anti-diabetic and antioxidant potential of Palmaria palmata protein hydrolysates
Harnedy, PA,FitzGerald, RJ (2013) In vitro assessment of the cardioprotective, anti-diabetic and antioxidant potential of Palmaria palmata protein hydrolysates. Journal Of Applied Phycology :1793-1803
Bioactive peptides from marine processing waste and shellfish: A review
Harnedy, PA,FitzGerald, RJ (2012) Bioactive peptides from marine processing waste and shellfish: A review. Journal Of Functional Foods :6-24
Isolation, purification and characterization of antioxidant peptidic fractions from a bovine liver sarcoplasmic protein thermolysin hydrolyzate
Di Bernardini, R; Rai, DK; Bolton, D; Kerry, J; O'Neill, E; Mullen, AM; Harnedy, P; Hayes, M (2011) Isolation, purification and characterization of antioxidant peptidic fractions from a bovine liver sarcoplasmic protein thermolysin hydrolyzate. Peptides :388-400
Antioxidant and antimicrobial peptidic hydrolysates from muscle protein sources and by-products
Di Bernardini, R., Harnedy, P.A., Bolton, D., Kerry, J., O'Neill, E., Mullen, A. M., & Hayes, M. (2011) Antioxidant and antimicrobial peptidic hydrolysates from muscle protein sources and by-products. Food Chemistry :1296-1307
Bioactive proteins, peptides and amino acids from macroalgae
Harnedy, PA; FitzGerald, RJ (2011) Bioactive proteins, peptides and amino acids from macroalgae. Journal Of Phycology :218-232
The cyanide hydratase enzyme of Fusarium lateritium also has nitrilase activity
Nolan, LM; Harnedy, PA; Turner, P; Hearne, AB; O'Reilly, C. (2003) The cyanide hydratase enzyme of Fusarium lateritium also has nitrilase activity. Fems Microbiology Letters :161-165
Books
Book Chapters
Enzyme-Assisted Extraction of Plant Proteins.
Kleekayai, T., Khalesi, M., Amigo-Benavent, M., Cermeño, M., Harnedy-Rothwell, P., FitzGerald, R.J. (2023) Enzyme-Assisted Extraction of Plant Proteins.. Switzerland : Springer Green Protein Processing Technologies from Plants
Marine processing proteinaceous by-products: A source of biofunctional food ingredients'
Neves, A.C., Harnedy, P.A. & FitzGerald, R. J. (2016) Marine processing proteinaceous by-products: A source of biofunctional food ingredients' . UK : Academic Press Protein Byproducts: Transformation from environmental burden into value-added products.
Marine processing proteinaceous by-products: A source of biofunctional food ingredients.
Neves, A.C., Harnedy, P.A. & FitzGerald, R. J. (2016) Marine processing proteinaceous by-products: A source of biofunctional food ingredients.. UK : Academic Press Protein Byproducts: Transformation from environmental burden into value-added products. :63-86
Extraction and enrichment of protein from red and green macroalgae.
Harnedy, PA; FitzGerald, RJ (2015) Extraction and enrichment of protein from red and green macroalgae.. New York : Springer Natural Products from Marine Algae, Methods in Molecular Biology :103-108
Antihypertensive peptides from marine origin.
Norris, R; Harnedy, PA; FitzGerald, RJ (2013) Antihypertensive peptides from marine origin.. Chichester, UK. : John Wiley & Sons Ltd. Bioactive Compounds from Marine Foods: Plant and Animal Sources :27-56
Bioactive proteins and peptides from macroalgae, fish, shellfish and marine processing waste
Harnedy, PA; FitzGerald (2013) Bioactive proteins and peptides from macroalgae, fish, shellfish and marine processing waste. Chichester, UK : John Wiley & Sons, Ltd. Marine Proteins and Peptides: Biological Activities and Applications :5-39
Edited Books
Other Journals
Conference Publications
Conference Contributions
Published Reports
Editorials
Book Reviews
Other Publications
BIOACTIVE PROTEINS, PEPTIDES, AND AMINO ACIDS FROM MACROALGAE
Harnedy, PA; FitzGerald, RJ (2011) BIOACTIVE PROTEINS, PEPTIDES, AND AMINO ACIDS FROM MACROALGAE. :218-232